High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. Bright-Glo™ Luciferase Assay System is designed specifically to meet the needs of continuous-process systems by providing robust, homogeneous assay chemistry that achieves high assay sensitivity and approximately 30-minute signal half-life without prior sample processing. The assay is also less sensitive to mixing conditions, sample evaporation and pipetting errors. These attributes also benefit those who are using fewer samples but still require high sensitivity and ease of use.

Assay Advantages

• No sample preprocessing. With the Bright-Glo Assay, there is  no need to remove culture medium or wash cells prior to adding assay reagent. Grow and assay cells directly in the same multiwell plate.
• High Sensitivity. Up to tenfold more sensitive than other homogenous luciferase assay reagents.
• Convenient and Easy to Use. Simply mix buffer with lyophilized substrate and add to cells in culture medium; no need to thaw or measure before use.